SIRP alpha (SIRPA) Mouse Monoclonal Antibody [Clone ID: 15-414]

Specifications

Product Data
Clone Name	15-414
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 1x106 cells in 100 µl.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Monocyte-derived dendritic cells.
Specificity	This antibody is specific for CD172a, also known as signal-regulatory protein alpha.
Formulation	PBS Label: PE State: Lyophilized purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Reconstitution Method	Reconstitute with 1.0ml distilled water.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	PE
Storage Condition	Prior to and following reconstitution store undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	signal regulatory protein alpha
Database Link	Entrez Gene 140885 Human UniProt ID P78324
Background	CD172a is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
Synonyms	SHP substrate 1, SHPS-1, Sirp-alpha-2, Sirp-alpha-3, MyD-1 antigen, p84, BIT, MFR, MYD1, PTPNS1, SHPS1
Reference Data

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