Tfrc Rat Monoclonal Antibody [Clone ID: 8D3]

Specifications

Product Data
Clone Name	8D3
Applications	FC, FN, IHC
Recommend Dilution	Functional Assays. Dilute 1/100 - 1/200 Flow Cytometry: Use 10 µl of 1/100-1/200 diluted CD71 antibody to label 106 cells. Immunohistochemistry on Frozen Sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse transformed endothelioma cell line t.end1. Spleen cells from immunised Lewis rats were fused with the cells of the mouse NSO myeloma cell line
Specificity	This antibody recognises CD71, a 95kD cell surface protein that is otherwise known as transferrin receptor. This antibody recognises native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells. The antibody has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the Mouse.
Formulation	Phosphate buffered saline without preservatives Endotoxin Level: < 0.01 Eu/µg State: Low Endotoxin State: Liquid purified IgG fraction Stabilizer: None Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	transferrin receptor
Database Link	Entrez Gene 22042 Mouse UniProt ID Q62351
Background	CD71 is a major iron-binding protein, which plays a key role in the transport of iron into cells that require it. In mice, CD71 is widely expressed on a variety of cells, including Sertoli cells and cells which form the blood brain barrier (BBB) in the central nervous system.
Synonyms	TfR1, p90, Transferrin receptor protein 1
Reference Data

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