Cd80 Rat Monoclonal Antibody [Clone ID: RM80]

Specifications

Product Data
Clone Name	RM80
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 ul of neat antibody to label 1x10e6 cells in 100 ul.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	BCL1 cells expressing CD80.
Specificity	This antibody recognises mouse CD80 (B7-1), a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA + 5% sucrose as stabilizer. Label: PE State: Lyophilized purified IgG fraction. Label: R. Phycoerythrin (RPE)
Reconstitution Method	Reconstitute with 1.0 ml distilled water.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Name	CD80 antigen
Database Link	Entrez Gene 12519 Mouse UniProt ID Q00609
Background	CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
Synonyms	CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1
Reference Data

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