Caspase 8 (CASP8) Mouse Monoclonal Antibody [Clone ID: FLICE 3]

Specifications

Product Data
Clone Name	FLICE 3
Applications	WB
Recommend Dilution	Western Blot. Positive Control: MCF-7 cell lysates.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-8 protein and mouse myeloma cells.
Specificity	This antibody detects proenzyme form of human Caspase-8 by Western blot analysis as well as one of the cleavage products.
Formulation	PBS containing 0.08% Sodium Azide as preservative. State: Purified State: Liquid (sterile filtered) purified IgG fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	caspase 8
Database Link	Entrez Gene 841 Human UniProt ID Q14790
Background	Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-8 is a key enzyme in the apoptosis pathway. Caspase-8 contains two N-terminal region death effector domains which are removed to activate the enzyme along with cleavage into the two subunits. These subunits then for the active protease which is capable of cleaving caspase-3, -6 and -7. These caspases are then capable of cleaving other cellular substrates such as PARP and DFF, which in turn induce apoptosis.
Synonyms	CASP-8, CASP8, MCH5, CAP4
Reference Data

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