CDK7 Mouse Monoclonal Antibody [Clone ID: MO1]

Specifications

Product Data
Clone Name	MO1
Applications	IHC, IP, WB
Recommend Dilution	Western Blot (1-10 µg/ml). Immunoprecipitation. Immunohistochemistry on Frozen Sections. Recommended positive control tissue: HeLa cells fixed with acetone/methanol.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant 221 amino acid fragment of the C terminus of p40mo15 recognizing a 40 kD protein.
Specificity	This Cdk7 antibody, clone MO-1 recognizes Human Cyclin-dependent kinase 7.
Formulation	PBS State: Purified State: Liquid (sterile filtered) purified IgG fraction. Preservative: 0.08% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A/G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cyclin-dependent kinase 7
Database Link	Entrez Gene 1022 Human UniProt ID P50613
Background	It plays a crucial role in the activation of cdk’s. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Synonyms	CAK1, P39 Mo15, STK1, CDK-activating kinase
Reference Data

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