IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: 1TYV]

Specifications

Product Data
Clone Name	1TYV
Applications	FC
Recommend Dilution	Flow Cytometry. Monitoring of activated T cells in peripheral blood. Analysis of NK subsets. Study of B cell activation.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	CD25=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with PHA-activated human T-lymphocytes.
Specificity	Identification of human receptor for Interleukin-2 (IL-2R) expressing the 55,000 M.W. surface antigen. This antibody is specific for Interleukin-2 Receptor Lymphocytes.
Formulation	PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative. Label: APC State: Liquid purified IgG fraction.
Concentration	lot specific
Conjugation	APC
Storage Condition	Store the antibody undiluted at 2-8°C. Do Not Freeze!
Gene Name	interleukin 2 receptor subunit alpha
Database Link	Entrez Gene 3559 Human UniProt ID P01589
Background	The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.
Synonyms	Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
Note	Protocol: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Reference Data

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