ICAM1 Mouse Monoclonal Antibody [Clone ID: 1H4]

Specifications

Product Data
Clone Name	1H4
Applications	FC, IF, IHC, WB
Recommend Dilution	Flow Cytometry: 1 µg/ml. Immunocytochemistry. Western blot. Immunohistochemistry on Frozen Sections: 5-10 µg/ml. Immunohistochemistry on Paraffin Sections: 10 µg/ml Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Raji cells and spleen cells fused with NS1 cells
Specificity	The antibody reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Formulation	Azide free PBS preserved by filter sterilization, pH~7.4 State: Azide Free State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	intercellular adhesion molecule 1
Database Link	Entrez Gene 3383 Human UniProt ID P05362
Background	CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Synonyms	ICAM-1
Reference Data

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