Nitrotyrosine Mouse Monoclonal Antibody [Clone ID: 39B6]

Specifications

Product Data
Clone Name	39B6
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA (1,6). Immunoprecipitation. Immunofluorescence. Western blot (1): 0.7 µg/ml was sufficient for detection of 5 µg SN-1 treated BSA using Goat anti-Mouse IgG-HRP as the secondary antibody. Immunohistochemistry on Frozen Sections (1,5).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	3-(4-Hydroxy-3-Nitrophenyl Acetamido) Propionic Acid-BSA conjugate
Specificity	Recognizes 3-Nitrotyrosine moieties. No detectable cross-reactivity with non-Nitrated Tyrosine. Not species specific.
Formulation	PBS containing 0.09% Sodium Azide as preservative in 50% Glycerol. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an mportant component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, sinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high orrelation to disease states such as atherosclerosis, Alzheimer' disease, Parkinson’s disease and amyotrophic lateral sclerosis (4).
Synonyms	NO-Tyrosine, Nitro-Tyrosine
Reference Data

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