AHNAK Mouse Monoclonal Antibody [Clone ID: EM-09]

Specifications

Product Data
Clone Name	EM-09
Applications	IF, IHC, IP, WB
Recommend Dilution	Western Blot. Positive Control: HeLa Human cervix carcinoma cell line. Immunocytochemistry (Permeabilization is required). Positive Control: HeLa Human cervix carcinoma cell line. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially expressed fragment of N-terminal domain of Human AHNAK1
Specificity	The antibody reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	AHNAK nucleoprotein
Database Link	Entrez Gene 79026 Human UniProt ID Q09666
Background	AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.
Synonyms	Desmoyokin
Reference Data

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