HLA class II DR beta 1 / HLA-DRB1 Mouse Monoclonal Antibody [Clone ID: MEM-267]

Specifications

Product Data
Clone Name	MEM-267
Applications	ELISA, FC, WB
Recommend Dilution	Western Blotting. Flow Cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms. ELISA.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.
Specificity	The antibody MEM-267 specifically binds to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Formulation	PBS, pH 7.4 State: Aff - Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P04229
Background	HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
Synonyms	MHC class II antigen DRB1, DR1, DR-1
Reference Data

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