BMP4 Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IF, WB
Recommend Dilution	ELISA: In a Sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 2000 pg/ml of recombinant Human BMP-4 when used in conjunction with compatible secondary reagents. Western Blot: To detect Human BMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP-4 (5.0 ng/ml), a concentration of 0.4-0.6 μg/ml of this antibody is required. Immunofluorescence: This antibody stained HepG2 cells. The primary antibody was incubated at 3.0 µg/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A HeLa cell-derived recombinant Human BMP-4
Specificity	This antibody detects BMP4.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	The lyophilized antibody is stable at room temperature for one month or at -20°C for longer. Following reconstitution it is stable at 2-8°C for six weeks. Avoid repeated freezing and thawing.
Gene Name	bone morphogenetic protein 4
Database Link	Entrez Gene 652 Human UniProt ID P12644
Background	BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.
Synonyms	BMP2B, BMP-2B, BMP-4, DVR4, Bone morphogenetic protein 4
Note	Centrifuge vial prior to opening.
Reference Data

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