IL11 Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-11 (5.0 ng/ml), a concentration of 2.4-3.6 µg/ml is required. Sandwich ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 8-9 µg/ml will detect at least 0.2 ng/well of recombinant Human IL-11 when used with Biotin anti-Human IL-11 antibody (Cat.No PP1033B) as the detection antibody at a concentration of at least 2 µg/ml. Western Blot: To detect Human IL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human IL-11
Specificity	This antibody detects Human Interleukin-11. Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified IgG fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	interleukin 11
Database Link	Entrez Gene 3589 Human UniProt ID P20809
Background	Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production. Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.
Synonyms	IL-11, Adipogenesis inhibitory factor, AGIF
Reference Data

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