CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: DJ130c]

Specifications

Product Data
Clone Name	DJ130c
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/20-1/200 diluted antibody to label 1x106 cells in 100 µl. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	CD34 antigen
Specificity	This antibody recognizes Human CD16, a 50-65kD cell surface molecule. It recognizes all polymorphonuclear cells irrespective on their NA phenotype. Studies show that the DJ130c antibody recognizes an epitope in the first membrane-distal domain of CD16 and recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from Rhesus macaques, Macaca mulatta (Xu et al. 2012).
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Fc fragment of IgG receptor IIIa
Database Link	Entrez Gene 2214 Human UniProt ID P08637
Background	CD16 which is the low affinity receptor for IgG (FcRIII), exists as a transmembranous form (Fc gammaRIIIA, or CD16a) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16b). CD16a is expressed by NK cells, some T cells, and macrophages, whereas CD16b is primarily expressed by granulocytes. In addition, CD16b exists as two allelic variants NA1 and NA2.
Synonyms	FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2
Reference Data
Protein Families	ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways	Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, Systemic lupus erythematosus

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