TNFRSF1B Mouse Monoclonal Antibody [Clone ID: 2/220]

Specifications

Product Data
Clone Name	2/220
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Western Blot: 1-2 µg/ml. Using the concentration stated the detection limit is 10 ng/lane (reducing conditions). Immunohistochemistry on Frozen Sections: 15-25 µg/ml. ELISA: Using the concentrations stated the detection limits is 5 ng/well. Functional Assays.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	R-DNA
Specificity	Reacts with recombinant and native soluble Human TNF RII. This antibody neutralises the biological effect of rhTNF RII, but not rhTNF RI.
Formulation	PBS containing without preservatives or stabilizers State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Name	tumor necrosis factor receptor superfamily member 1B
Database Link	Entrez Gene 7133 Human UniProt ID P20333
Background	Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.
Synonyms	Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, TNFRSF1B, TNFBR, TNF-R2
Note	Endotoxin level: < 0.1 EU per 1 µg of the antibody, as determined by the LAL method.
Reference Data

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