MICA (+ MICB) Mouse Monoclonal Antibody [Clone ID: 6D4]

Specifications

Product Data
Clone Name	6D4
Applications	FC, FN, IHC, IP
Recommend Dilution	Immunoprecipitation. Functional Assays. Flow Cytometry: Use 10µl of the suggested working dilution to label 1x10e6 cells in 100µl. Immunohistochemistry on frozen sections: 1/100.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	MICA transfected C1R cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the P3 mouse myeloma cell line.
Specificity	This antibody is specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB).
Formulation	PBS, pH 7.4 State: Low Endotoxin State: Liquid purified Ig
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	MHC class I polypeptide-related sequence A
Database Link	Entrez Gene 100507436 Human UniProt ID Q29983
Background	MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.
Synonyms	MHC class I polypeptide-related sequence A, MIC-A, PERB11.1
Note	Endotoxin Level: less than 0.01 EU/mg
Reference Data

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