CD44 Mouse Monoclonal Antibody [Clone ID: Bu52]

Specifications

Product Data
Clone Name	Bu52
Applications	FC, IHC, IP
Recommend Dilution	Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/25-1/100 diuted antibody to label 1x10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/100-1/200 (Requires antigen retrieval using heat treatment with citrate buffer pH 6.0 prior to staining of paraffin sections).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognises the CD44 cell surface antigen, a 100kD glycoprotein.
Formulation	PBS, pH 7.4, containing 0.09% Sodium Azide State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD44 molecule (Indian blood group)
Database Link	Entrez Gene 960 Human UniProt ID P16070
Background	CD44 is widely expressed on human leucocytes,white matter of the brain and by some epithelial cells of the intestine and of the breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. The Bu52 antibody recognises an epitope that is common to all CD44 isoforms. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
Synonyms	LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1
Reference Data

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