Tfrc Rat Monoclonal Antibody [Clone ID: R17217.1.4]

Specifications

Product Data
Clone Name	R17217.1.4
Applications	FC
Recommend Dilution	Flow Cytometry.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	Anti-mouse CD71 antigen monoclonal antibody reacts with the transferrin receptor (CD71). The expression of CD71 is up-regulated after B cell or T cell activation.
Formulation	PBS containing 0.09% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein Label: Biotin State: Liquid purified Ig fraction
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	transferrin receptor
Database Link	Entrez Gene 22042 Mouse UniProt ID Q62351
Synonyms	TfR1, p90, Transferrin receptor protein 1
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1.0 μg of this antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 μl of secondary antibody (Streptavidin-FITC) at a 1/500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 μl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: (Representative Histogram) Mouse Strain: Mouse Hybridomas Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 1.0 μg/10e6 cells Isotypic Control: Biotin Rat IgG2a
Reference Data

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