MDM2 (154-167) Mouse Monoclonal Antibody [Clone ID: SMP14]

Specifications

Product Data
Clone Name	SMP14
Applications	IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Immunohistochemistry on Frozen and Resin Sections. Immunohistochemistry on Paraffin Sections: 1/200-1/500. Antigen retrieval using heat treatment prior to staining of paraffin sections is required. EDTA pH 8.0 is recommended for this purpose. Recommended Positive Control: Breast Carcinoma.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	A Synthetic peptide corresponding to amino acids 154-167 of Human MDM2 protein. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Specificity	This antibody recognizes the 90kD MDM2 protein, which forms a complex with p53 leading to p53 inhibition. May also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer. Cellular Localization: nuclear.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	MDM2 proto-oncogene
Database Link	Entrez Gene 4193 Human UniProt ID Q00987
Background	Human homolog of Murine Double Minute-2 (MDM2) and p53 function in mutual regulation: activation of p53 results in expression of MDM2 which consequently inhibits the ability of wild type p53 to stimulate expression of target genes. MDM2 is frequently overexpressed in the nuclei of soft tissue sarcomas, and to a much lesser extent, in a wide variety of malignancies of bladder, breast, testes, cervix, and glial cells.
Synonyms	p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, Hdm2
Note	Predicted Molecular weight: 55 kDa.
Reference Data

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