STAT3 pSer727 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 23G5]

Specifications

Product Data
Clone Name	23G5
Applications	ELISA, IHC, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunohistochemistry. Included Positive Control: Cell lysate from HepG2 pervanadate treated.  Format: Lyophilized cell lysate from HepG2 cells.        Serum starved cells were treated for 15min with pervanadate. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjuagted to KLH.  Epitope: ...L P M pS F R T...
Specificity	This antibody specifically recognizes STAT3 phosphorylated at Ser727. The antibody does not crossreact with the non-phosphorylated form of STAT3 nor with unrelated serine-phosphorylated proteins.
Formulation	1ml 2 x PBS with 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size	92 kDa
Gene Name	signal transducer and activator of transcription 3
Database Link	Entrez Gene 6774 Human UniProt ID P40763
Background	The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. Besides tyrosine phosphorylation, STAT3 activity is regulated by serine phosphorylation at serine 727. Recent reports indicate that both MAP kinase and SAP kinase induce phosphorylaton at serine 727.
Synonyms	STAT-3, Acute-phase response factor, APRF
Reference Data

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