SHC (SHC1) pSer36 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6E10]

CAT#: AM00144PU-N

SHC (SHC1) pSer36 (incl. pos. control) mouse monoclonal antibody, clone 6E10, Purified



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 6E10
Applications ELISA, WB
Recommend Dilution Western Blot: 1 µg/ml for HRPO/ECL detection.
Recommended blocking buffer BPPT:  1% (w/v) BSA, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1%(v/v) Tween 20, 2XPBS.
Reactivity Canine, Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Synthetic phosphopeptide conjugated to KLH
Epitope: E-L-P-pS-P-S-A
Specificity This antibody specifically recognizes shc/p66 when it is phosphorylated at Serine 36. We recommend to Immunoprecipitate shc/p66 prior to detection with mab shc/p66-6E10.
Formulation 1 ml PBS
State: Purified
State: Lyophilized purified IgG fraction
Stabilizer: PEG and Sucrose
Preservative: 0.09% Sodium Azide
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Predicted Protein Size 66 kDa
Gene Name SHC adaptor protein 1
Background Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signalling pathways.
Synonyms SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3, SHCA
Note Protocol: Included Positive Control Cell Lysate: HepG2 shc/p66-IP

Formulation: shc/p66 immunoprecipitated with anti-shc (BD Transduction Laboratories) from PMA stimulated HepG2 cells. Proteins were eluted with SDS and lyophilized from PBS/SDS.

Reconstitution: Restore by addition of 125 µl H2O. After complete solubilization add 125 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
Aliquote and store frozen. Avoid repeated freezing and thawing.

Application: The positive control cell lysate is recommended for immunoblot applications. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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