Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2A5]

CAT#: AM00123PU-N

Phosphotyrosine (incl. pos. control) mouse monoclonal antibody, clone 2A5, Purified

Conjugation: Unconjugated Biotin



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CNY 5,148.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 2A5
Applications ELISA, IP, WB
Recommend Dilution ELISA: Use at 0.1 µg/ml.
Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
Immunoprecipitation: Use at 1-10 µg per 10e6 pervanadate-treated A431 cells.
Included Positive Control: Phosphotyrosine MW standard.
Reactivity Canine, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Synthetic phosphopeptide conjugated to KLH
Epitope:...E-pY-M...
Specificity This monoconal antibody (2A5) recognizes a broad spectrum of Tyrosine phosphorylated proteins in crude cell extracts.
It tolerates a negative charge N-terminal to the Phosphotyrosine residue.
Formulation 1 ml 2 x PBS with 0.09% Sodium Azide/PEG and Sucrose.
State: Purified
State: Lyophilized purified IgG fraction.
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Background Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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