Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2A5]

Specifications

Product Data
Clone Name	2A5
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: Use at 0.1 µg/ml. Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: Use at 1-10 µg per 10e6 pervanadate-treated A431 cells. Included Positive Control: Phosphotyrosine MW standard.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH Epitope:...E-pY-M...
Specificity	This monoconal antibody (2A5) recognizes a broad spectrum of Tyrosine phosphorylated proteins in crude cell extracts. It tolerates a negative charge N-terminal to the Phosphotyrosine residue.
Formulation	1 ml 2 x PBS with 0.09% Sodium Azide/PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Background	Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.
Reference Data

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