Phosphoserine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 4H4]

Specifications

Product Data
Clone Name	4H4
Applications	ELISA, IP, WB
Recommend Dilution	Western Blot: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILK OR CASEIN FOR BLOCKING! ELISA: 0.05 µg/ml. Immunoprecipitation: 1-10 µg per 10e6 pervanadate-treated A431 cells. Included Positive Control: Phosphoserine/phosphothreonine positive control.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH
Specificity	This antibody recognizes a broad range of Serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighboured to Phosphoserine. Please note that Phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence!
Formulation	1ml 2xPBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fractio from Serum-free cell culture supernatant
Reconstitution Method	Restore with 1.0 ml H2O (15 min, RT).
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.
Reference Data

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