AKT1 (specific) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5G12]

CAT#: AM00108PU-N

AKT1 (specific) (incl. pos. control) mouse monoclonal antibody, clone 5G12, Purified



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 5G12
Applications ELISA, IHC, WB
Recommend Dilution ELISA: Use at 0.05 µg/ml.
Western Blot: Use at 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Positive Control: Cell lysate from untreated Neuro2A cells.
The antiboby crossreacts in immunoblot applications with an unknown protein of ca. 120kDa expressed in human tumor cell lines.
Immunohistochemistry.
Reactivity Canine, Human, Rat
Host Mouse
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to KLH.
Epitope: aa 107-122
Specificity This antibody specifically recognizes the alpha isoform of PKB (PKBα/Akt1).
Formulation PBS containing 0.09% Sodium Azide, PEG and Sucrose/50% Glycerol
State: Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Predicted Protein Size 60 kDa
Gene Name AKT serine/threonine kinase 1
Background The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.
Synonyms Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT
Note Protocol: Positive Control Cell lysate provided
Neuro 2A untreated

Formulation: Lyophilized cell lysate from serum starved neuro 2A cells.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage: Store in aliquots at -20°C. Avoid repeated freezing and thawing.

Applications: The Positive Control Cell Lysate is recommended for Immunoblotting. 20 µl of positive cell lysate correspond to ca. 20.000 cells.
Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.

Note: The lyophilized cell lysate contains SDS and are not recommended for applications with native proteins such as Immunoprecipitation.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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