AKT1 (specific) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5G12]
CAT#: AM00108PU-N
AKT1 (specific) (incl. pos. control) mouse monoclonal antibody, clone 5G12, Purified
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CNY 5,973.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | 5G12 |
Applications | ELISA, IHC, WB |
Recommend Dilution | ELISA: Use at 0.05 µg/ml. Western Blot: Use at 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated Neuro2A cells. The antiboby crossreacts in immunoblot applications with an unknown protein of ca. 120kDa expressed in human tumor cell lines. Immunohistochemistry. |
Reactivity | Canine, Human, Rat |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Synthetic peptide conjugated to KLH. Epitope: aa 107-122 |
Specificity | This antibody specifically recognizes the alpha isoform of PKB (PKBα/Akt1). |
Formulation | PBS containing 0.09% Sodium Azide, PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified Ig fraction |
Concentration | lot specific |
Purification | Subsequent Thiophilic Adsorption and Size Exclusion Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Predicted Protein Size | 60 kDa |
Gene Name | AKT serine/threonine kinase 1 |
Database Link | |
Background | The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway. |
Synonyms | Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT |
Note | Protocol: Positive Control Cell lysate provided Neuro 2A untreated Formulation: Lyophilized cell lysate from serum starved neuro 2A cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Store in aliquots at -20°C. Avoid repeated freezing and thawing. Applications: The Positive Control Cell Lysate is recommended for Immunoblotting. 20 µl of positive cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysate contains SDS and are not recommended for applications with native proteins such as Immunoprecipitation. |
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