Nitrotyrosine Mouse Monoclonal Antibody [Clone ID: 7A5]

Specifications

Product Data
Clone Name	7A5
Applications	ELISA, WB
Recommend Dilution	Immunoblotting: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	KLH modified with Nitrotyrosine
Specificity	This antibody 7A5 recognizes Nitrotyrosine in proteins.
Formulation	1ml 2 x PBS State: Purified State: Lyophilized purified IgG fraction Stabilizer: PEG and Sucrose Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore with 1ml H2O (15 min, RT)
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.  Avoid repeated freezing and thawing.
Background	The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.
Synonyms	NO-Tyrosine, Nitro-Tyrosine
Reference Data

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