p38 (MAPK14) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2F2]

Specifications

Product Data
Clone Name	2F2
Applications	ELISA, IP, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive Control: Cell lysate from untreated A431 cells. ELISA: 0.05 µg/m. Immunoprecipitation: 1 - 10 µg per 10e6 pervanadate-treated A431 cells.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH
Specificity	This antibody is directed against the N-terminus of Mxi2.
Formulation	1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	mitogen-activated protein kinase 14
Database Link	Entrez Gene 1432 Human UniProt ID Q16539
Background	Mxi2 is a splice variant of SAPK2α (p38). Stress-activated protein kinases (SAPK) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines.
Synonyms	Mitogen-activated protein kinase 14, p38 alpha, MXI2, SAPK2A, CSBP, CSBP1, CSBP2, CSPB1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.