MEK7 (MAP2K7) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10F7]

Specifications

Product Data
Clone Name	10F7
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA: 0.1 µg/ml. Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from Untreated SW480 cells. Immunocytochemistry. Immunohistochemistry on Frozen Sections.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH.  Epitope: N-Terminus.
Specificity	This antibody specifically recognizes the N-terminus of MKK7.
Formulation	1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction from Serum-free Cell Culture Supernatant
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Predicted Protein Size	45 kDa
Gene Name	mitogen-activated protein kinase kinase 7
Database Link	Entrez Gene 5609 Human UniProt ID O14733
Background	The stress response of mammalian cells results in activation of kinases of the SAPK1 and SAPK2 families. The SAP kinases are specifically activated by dual phosphorylation of a conserved T-X-Y motif by MAP kinase kinases (MKK). The 3 isoforms of the SAPK1 family (SAPK1α/jnk2; SAPK1β/jnk3; SAPK1γ/jnk1) are synergistically activated by phosphorylation of the threonine residue by MKK7 and the tyrosine residue by MKK4.
Synonyms	MAPK/ERK kinase 7, JNK-activating kinase 2, c-Jun N-terminal kinase kinase 2, MAPKK 7, NKK2, MEK7, MKK7, MAP kinase kinase 7
Note	Protocol: Positive Control Cell lysate from untreated SW480 cells Format: Lyophilized cell lysate from serum starved SW480 cells. Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDSPAGE sample buffer, mix and incubate at 90°C for 5 min. Applications: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 μl / lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation. Storage: In aliquots at -20°C. Avoid repeated freezing and thawing.
Reference Data

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