MEK1 (MAP2K1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9G3]

Specifications

Product Data
Clone Name	9G3
Applications	ELISA, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Included Positive Control: Cell lysate from untreated HepG2 cells. (See Protocols) Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml (protein ELISA).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH
Specificity	This antibody specifically recognizes the activation loop of MEK1/2 independent of its phosphorylation status.
Formulation	1 ml PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified Ig
Reconstitution Method	Restore with 1 ml H2O (15 min, RT)
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase kinase 1
Database Link	Entrez Gene 26395 MouseEntrez Gene 170851 RatEntrez Gene 5604 Human UniProt ID Q02750
Background	MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase on both threonine and tyrosine residues of the activation motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.
Synonyms	MAP2K1 - mitogen-activated protein kinase kinase 1, MAPKK1, MEK1, MKK1, PRKMK1, MAP2K2 - mitogen-activated protein kinase kinase 2, MAPKK2, MEK2, MKK2, PRKMK2
Note	Protocol: Positive Control: Cell lysate from untreated HepG2 cells Formulation: Lyophilized cell lysate from serum starved HepG2 cells Reconstitution: Reconstitute by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway

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