MEK1 (MAP2K1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7E10]

CAT#: AM00090PU-N

MEK1 (MAP2K1) (incl. pos. control) mouse monoclonal antibody, clone 7E10, Purified



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name 7E10
Applications ELISA, WB
Recommend Dilution Suitable for Western Blot (0.5 μg/ml for HRPO/ECL detection)
Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Use SW480 pervanadate-treated as positive control (see "Protocols" below).
ELISA: 0.1 µg/ml.
Reactivity Canine, Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Phosphopeptide conjugated to KLH.
Epitope: pSer 218/222 in MEK1 pSer 222/226 in MEK2
Specificity AM00090PU-N specifically recognizes MEK1 phosphorylated at serine 218/222 and MEK2 phosphorylated at serine 222/226.
Formulation 1 x PBS / 0.09 % Na-azide / PEG and Sucrose
State: Purified
State: Lyophilized
Reconstitution Method Reconstitute with 1 ml H2O (15 min, RT)
Purification Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Conjugation Unconjugated
Storage Condition For long-term storage, freeze lyophilizate upon arrival (-20°C).
Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Gene Name mitogen-activated protein kinase kinase 1
Background MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase 1 and 2 on both threonine and tyrosine residues of the activation loop motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.
Synonyms MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1
Note A positive control cell lysate is provided with this antibody.

Protocol: Contains Positive Control (pervanadate treated SW 480 cells)

Format: Lyophilized cell lysate from SW480 cells. Serum starved cells were treated for 15min with pervanadate.

Reconstitution: Add 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl/lane (mini gel) for HRP/ECL detection of the target proteins.

Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.

Aliquot and store frozen. Avoid repeated freeze/thaw cycles.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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