MEK1 (MAP2K1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7E10]

Specifications

Product Data
Clone Name	7E10
Applications	ELISA, WB
Recommend Dilution	Suitable for Western Blot (0.5 μg/ml for HRPO/ECL detection) Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use SW480 pervanadate-treated as positive control (see "Protocols" below). ELISA: 0.1 µg/ml.
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH. Epitope: pSer 218/222 in MEK1 pSer 222/226 in MEK2
Specificity	AM00090PU-N specifically recognizes MEK1 phosphorylated at serine 218/222 and MEK2 phosphorylated at serine 222/226.
Formulation	1 x PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized
Reconstitution Method	Reconstitute with 1 ml H2O (15 min, RT)
Purification	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Gene Name	mitogen-activated protein kinase kinase 1
Database Link	Entrez Gene 5604 Human UniProt ID Q02750
Background	MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase 1 and 2 on both threonine and tyrosine residues of the activation loop motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.
Synonyms	MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1
Note	A positive control cell lysate is provided with this antibody. Protocol: Contains Positive Control (pervanadate treated SW 480 cells) Format: Lyophilized cell lysate from SW480 cells. Serum starved cells were treated for 15min with pervanadate. Reconstitution: Add 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRP/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation. Aliquot and store frozen. Avoid repeated freeze/thaw cycles.
Reference Data

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