Insulin Receptor (INSR) (beta chain) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11B6]

CAT#: AM00078PU-N

Insulin Receptor (INSR) (beta chain) (incl. pos. control) mouse monoclonal antibody, clone 11B6, Purified



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name 11B6
Applications ELISA, WB
Recommend Dilution ELISA: 0.1 µg/ml.
Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Positive Control Provided: Cell lysate from untreated HepG2.
Formulation: Lyophilized cell lysate from serum starved HepG2 cells.
Reconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
Aliquote and store frozen. Avoid repeated freezing and thawing. 
Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells.
Use 20µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reactivity Canine, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to KLH
Specificity This antibody specifically recognizes the C-terminus of Insulin receptor (phosphorylation-independent).
Formulation 1ml 2 x PBS with 0.09% Sodium Azide, PEG and Sucrose
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subsequent thiophilic adsorption and size exclusion chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -20 to -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Predicted Protein Size 97 kDa
Gene Name insulin receptor
Background The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.
Synonyms Insulin Receptor
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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