Insulin Receptor (INSR) pTyr1322 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 21G12]

CAT#: AM00076PU-N

Insulin Receptor (INSR) pTyr1322 (incl. pos. control) mouse monoclonal antibody, clone 21G12, Purified



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name 21G12
Applications ELISA, WB
Recommend Dilution Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Positive Control: Cell lysate from pervanadate-treated HEK-293 cells.
ELISA: 0.1 µg/ml.
Reactivity Canine, Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Synthetic phosphopeptide conjugated to KLH. 
Epitope: Phosphotyrosine 1322 (HIPpYTHM)
Specificity This antibody specifically recognizes Insulin receptor phosphorylated at Tyrosine 1322.
Formulation 1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name insulin receptor
Background The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.
Synonyms Insulin Receptor
Note Protocol:  Positive Control: Cell lysate from pervanadate-treated HEK-293 cells. 

Format: Lyophilized cell lysate from HEK-293 cells. 
Serum starved cells were treated for 15 minutes with pervanadate.

Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Application: The positive control cell lysate is recommended for immunoblot applications.
20 μl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins.

Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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