HSP27 (HSPB1) pSer82 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5B9]

Specifications

Product Data
Clone Name	5B9
Applications	IHC, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from EGF-treated HepG2 cells (See Protocols for more details). Immunohistochemistry on Frozen Sections.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH. Epitope: R Q L pS S G V
Specificity	This antibody specifically interacts with HSP27 phosphorylated at Serine 82 and does not crossreact with the non-phosphorylated HSP27 nor with unrelated Serine-phosphorylated proteins.
Formulation	1 ml PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	heat shock protein family B (small) member 1
Database Link	Entrez Gene 3315 Human UniProt ID P04792
Background	The small heat shock protein hsp27 is constitutively expressed in most cell lines. The expression level is increased in response to environmental stress. Activation of the p38 kinases results in subsequent activation of MAPKAP2 that phosphorylates hsp27 at Ser15, Ser78 and Ser82.
Synonyms	Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25
Note	Protocol: Positive Control Cell Lysate: HepG2 EGF treated Format: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min with EGF. Reconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min.  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl positive control cell lysate correspond to approx. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysates contain SDS and are not recommended for applicatioins with native proteins such as Immunoprecipitation. Storage: Aliquot and store frozen. Avoid repeated freeze/thaw cycles.
Reference Data

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