GSK3 alpha (GSK3A) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10C1]

Specifications

Product Data
Clone Name	10C1
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Immunocytochemistry. Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for more details).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin
Specificity	This antibody specifically recognizes GSK3-alpha phosphorylation independent at 51 kDa. Mab GSK3a-10C1 does not crossreact with GSK3-beta.
Formulation	PBS containing 0.09% Sodium Azide/PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	51 kDa
Gene Name	glycogen synthase kinase 3 alpha
Database Link	Entrez Gene 2931 Human UniProt ID P49840
Background	Glycogen Synthase Kinase 3 (GSK3) exists in two isoforms, alpha and beta (GSK3alpha, GSK3beta). GSK3 is a serine/threonine kinase that becomes phosphorylated by PKB/akt upon activation of the PI3K signal transduction pathway. Phosphorylation of GSK3alpha at serine 21 or GSK3beta at serine 9, respectively, leads to inhibition of kinase activity of GSK3.
Synonyms	Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A
Note	Protocol: Positive Control Provided: Cell lysate from untreated SKOV-3 cells Format: Lyophilized cell lysate from serum starved SKOV-3 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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