c Fos (FOS) pSer374 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 34E4]

Specifications

Product Data
Clone Name	34E4
Applications	ELISA, IHC, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml (protein ELISA). Immunohistochemistry on frozen sections.
Reactivity	Canine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH.
Specificity	This antibody specifically interacts with c-Fos phosphorylated at serine 374.
Formulation	1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	Fos proto-oncogene, AP-1 transcription factor subunit
Database Link	Entrez Gene 2353 Human UniProt ID P01100
Background	The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.
Synonyms	FOS, G0S7
Reference Data

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