ErbB 3 (ERBB3) (Cytopl. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5A12]

Specifications

Product Data
Clone Name	5A12
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20. Included Positive Control: Cell lysate from untreaed SW620 cells. (See Protocols) Immunohistochemistry on Paraffin Sections. Immunocytochemistry: (1-10 µg/ml).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin (aa 1250-1270).
Specificity	This antibody recognizes erbB3 phosphorylation status independent at 185 kDa in Western blot.
Formulation	1 ml PBS State: Purified State: Lyophilized purified IgG fraction. Stabilizer: PEG and Sucrose Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Upon receipt, store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size	185 kDa
Gene Name	erb-b2 receptor tyrosine kinase 3
Database Link	Entrez Gene 2065 Human UniProt ID P21860
Background	ErbB3 is a member of the EGFR/erbB receptor tyrosine kinase family. ErbB3 lacks intrinsic kinase activity due to an amino acid substitution within the ATP binding site. Upon heterodimerization with the other erbB family members, erbB3 becomes phosphorylated at multiple tyrosine residues that represent docking sites for downstream signaling proteins, e.g. PI3K, grb7 and shc. The erbB3 cytoplasmic domain contains 6 docking sites for PI3K. Thus, erbB3 is a potent activator of the PI3K/PKB signaling pathway.
Synonyms	ERBB-3, c-erbB-3, HER-3
Note	Protocol: Positive Control: Cell lysate from untreated SW620 cells Formulation: Lyophilized cell lysate from serum starved SW 620 cells Reconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRP/ECL detetction of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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