EGFR (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13G8]

Specifications

Product Data
Clone Name	13G8
Applications	ELISA, IF, IP, WB
Recommend Dilution	ELISA (0.05 µg/ml). Immunoblotting: 1 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for more details). Immunoprecipitation (1-10 µg/ml per 10e6 pervanadate-treated A431 cells). Immunocytochemistry (1-10 µg/ml).
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to KLH. Epitope: C-terminus (aa 1165-1186) independent of phosphorylation status.
Specificity	This antibody specifically recognizes the C-terminus of EGF receptor (aa 1165 - 1186). Recognition is independent of the phosphorylation status at tyrosine 1173.
Formulation	PBS, 0.09% Sodium Azide/PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases. Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	Mol. weight: 180 kDa. Protocol: Positive Control Provided: Cell lysate from untreated HepG2 cells. Description: Cell lysate from untreated HepG2 cells. Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 μl H20. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 μl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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