EGFR pTyr1069 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11C2]

CAT#: AM00034PU-N

EGFR pTyr1069 (incl. pos. control) mouse monoclonal antibody, clone 11C2, Purified

Conjugation: Unconjugated Biotin



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 11C2
Applications ELISA, IP, WB
Recommend Dilution ELISA: Use at 0.05 µg/ml
Immunoblotting. 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Immunoprecipitation: Use at 1-10 µg per 10e6 pervanadate-treated A431 cells.
Positive Control: Cell lysate from pervanadate-treated HepG2 cells (See Protocols for more details).
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Phosphopeptide conjugated to KLH.
Epitope: Phosphotyrosine pY1069 (L Q R pY S S D).
Specificity This antibody specifically recognizes EGFR phosphorylated at Tyrosine 1069.
Formulation PBS, 0.09% Sodium Azide/PEG and Sucrose/50% Glycerol.
State: Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation Unconjugated
Storage Condition Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Gene Name epidermal growth factor receptor
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
The phosphorylation status of Tyrosine 1069 regulates the degradation of activated EGFR. Autophosphorylation of Tyrosine 1069 creates a docking site for the c-cbl ubiquitination ligase.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note Mol. weight: 180 kDa.

Protocol: Positive Control Provided.
Cell lysate from pervanadate-treated HepG2 cells


Format: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min with pervanadate.

Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage: Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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