EGFR pTyr1069 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11C2]

Specifications

Product Data
Clone Name	11C2
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml Immunoblotting. 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Immunoprecipitation: Use at 1-10 µg per 10e6 pervanadate-treated A431 cells. Positive Control: Cell lysate from pervanadate-treated HepG2 cells (See Protocols for more details).
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH. Epitope: Phosphotyrosine pY1069 (L Q R pY S S D).
Specificity	This antibody specifically recognizes EGFR phosphorylated at Tyrosine 1069.
Formulation	PBS, 0.09% Sodium Azide/PEG and Sucrose/50% Glycerol. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. The phosphorylation status of Tyrosine 1069 regulates the degradation of activated EGFR. Autophosphorylation of Tyrosine 1069 creates a docking site for the c-cbl ubiquitination ligase.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	Mol. weight: 180 kDa. Protocol: Positive Control Provided. Cell lysate from pervanadate-treated HepG2 cells Format: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min with pervanadate. Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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