EGFR pThr693 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5F10]

Specifications

Product Data
Clone Name	5F10
Applications	ELISA, WB
Recommend Dilution	ELISA: 0.1 µg/ml. Western blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from PMA-pervanadate-treated A431 cells (See Protocols).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to hemcyanin
Specificity	This antibody specifically recognizes EGFR phosphorylated at Threonine 693.
Formulation	PBS, 0.09% Sodium Azide, PEG and Sucrose, 50% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	Mol. weight: 180 kDa. Protocol: Positive Control: Cell lysate from PMA+pervanadate-treated A431 cells Description: Cell lysate from PMA+pervanadate-treated A431 cells, epidermoid carcinoma (Human) Format: Lyophilized cell lysate from A431 cells. Serum starved cells were treated for 15min with pervanadate plus PMA. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data

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