CREB1 pSer133/Ser63 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10E9]

Specifications

Product Data
Clone Name	10E9
Applications	ELISA, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml.
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH.
Specificity	This antibody specifically recognizes CREB phosphorylated at serine 133 and ATF1 phosphorylated at serine 63 in Western blot and ELISA applications.
Formulation	1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	cAMP responsive element binding protein 1
Database Link	Entrez Gene 1385 Human UniProt ID P16220
Background	The transcription factor CREB activates transcription in response to a variety of extracellular signals. Various kinases, including Protein kinase A (PKA) , and CaM kinase II, regulate CREB activity by phosphorylation of serine 133. Molecular Weight: CREB:43kDa; ATF1:38kDa
Synonyms	cAMP Response Element-Binding protein, CREB1, CREB-1
Reference Data

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