beta Catenin (CTNNB1) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10H8]

Specifications

Product Data
Clone Name	10H8
Applications	ELISA, IP, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 μg/ml. Immunoprecipitation: 1 - 10 μg per 10e6 vanadate treated A431 cells.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant β-Catenin
Specificity	This antibody specifically interacts with the C-terminus of β-catenin (aa 769 - 781).
Formulation	1 ml 2 x PBS / 0.1 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified Ig
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	catenin beta 1
Database Link	Entrez Gene 1499 Human UniProt ID P35222
Background	The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. β-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that β-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity.
Synonyms	CTNNB1, CTNNB, Beta-catenin
Reference Data

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