beta Catenin (CTNNB1) pTyr86 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24E1]

Specifications

Product Data
Clone Name	24E1
Applications	ELISA, WB
Recommend Dilution	Western Blot: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to hemocyanin
Specificity	This antibody specifically recognizes β-catenin phosphorylated at tyrosine 86 at 90 kDa.
Formulation	PBS/0.09% Na-Azide/PEG and Sucrose Label: Biotin State: Liquid purified IgG
Concentration	lot specific
Purification	Size exclusion chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody (aliquote in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	catenin beta 1
Database Link	Entrez Gene 1499 Human UniProt ID P35222
Background	The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. β-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that β-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity. Additionally, β-catenin is a substrate of both receptor and non-receptor tyrosine kinases. Tyrosine 86 and tyrosine 654 are substrates of EGF receptor and src family kinases while tyrosine 142 is a substrate of fer tyrosine kinase.
Synonyms	CTNNB1, CTNNB, Beta-catenin
Reference Data

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