BAD Mouse Monoclonal Antibody [Clone ID: 5E6]

Specifications

Product Data
Clone Name	5E6
Applications	IF, IP, WB
Recommend Dilution	Immunoprecipitation: 1-2 µg/ml. Also suitable for Western blotting if cells overexpressing BAD are used (0.5 µg/ml for HRPO/ECL detection). Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Immunocytochemistry. Note: We recommend to use mab BAD-5E6 for IP applications. The mab is also suitable for Western-blot applications if cells overexpressing BAD are used.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human BAD.
Specificity	This antibody specifically binds the BAD protein but does not recognize BAD/Bcl-2 heterodimers in Immunoprecipitation assays.
Formulation	1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiopilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freezing and thawing.
Gene Name	BCL2 associated agonist of cell death
Database Link	Entrez Gene 572 Human UniProt ID Q92934
Background	BAD is an important regulatory protein controlling cellsurvival/apoptosis. Unphosphorylated BAD heterodimerizes with Bcl-2 and Bcl-XL, thus neutralizing the anti-apoptotic properties of the Bcl proteins. Activation of different signal transduction pathways leads to phosphorylation of BAD at multiple serine residues, e.g. phosphorylation of serine 136 by PKB, serine 112 by MAPK, and serine 155 by PKA, and dissociation of the BAD/Bcl heterodimers.
Synonyms	BAD, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-L-8
Note	Molecular Weight. 19 kDa
Reference Data

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