Amyloid beta (1-40 specific) Mouse Monoclonal Antibody [Clone ID: 5C3]

Specifications

Product Data
Clone Name	5C3
Applications	ELISA, IF, WB
Recommend Dilution	ELISA: 0.1 μg/ml. Immunocytochemistry: 1-10 μg/ml. Western blot: 1 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	C-terminal peptide conjugated to KLH
Specificity	This antibody specifically interacts with the C-Terminus of beta-Amyloid (1-40) and does not crossreact with beta-Amyloid (1-42).
Formulation	PBS containing 0.09% Sodium Azide as preservative. Label: Biotin State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody (aliquote in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Background	The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).
Reference Data

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