Mouse IgG2a Isotype Control

Specifications

Product Data
sds_url	/sds/am26775le-n.pdf
_pt	2
immunogen	Mineral oils
_c	isotype-control
sub_category	Isotype Controls
applications_filter	FC, ICC/IF, IP, WB
antibody_host	Mouse
description	Mouse IgG2a Isotype Control
pdf_url	/datasheet/am26775le-n.pdf
concentration	1 mg/ml
isotypes_filter	IgG2a
storage	Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
product_name	Mouse IgG2a Isotype Control
clonality	Monoclonal
conjugates	Unconjugated
presentation_state	Low Endotoxin
background	The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
purification	Protein-A affinity chromatography
specificity	This antibody reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues. The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
buffer	Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
category	Antibodies
sizes_filter	100 ug

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.