Uba6 (NM_172712) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR211543 protein sequence Red=Cloning site Green=Tags(s) MERSEPLAVLSCEEASCSSWGACGASKNLPTMTTESLEIDDGLYSRQRYVLGDTAMQKMAKSCVFLSGMG GLGVEIAKNLVLAGIKALTIHDTKKCQAWDLGTNFFLCEDDVVNERNRAEAVLHRIAELNPYVQVSSSSA PLDETTDLSFLEKYQCVVLTEIKLTLQKKINNFCHSHCPPIKFISADVHGIWSRLFCDFGDEFEVSDTTG EEPKEIFISNITQANPGIVTCLESHPHKLETGQFLTFREIHGMTGLNGSVQQITVISPFSFSIGDTTKLD PYLHGGIAVQVKTPKTFCFEPLESQIKHPRCLIADFSKPEAPLEIHLAMLALDQFQENYNRKPNIRCQQD SDELLKLTVSINETLEEKPEVNADIVHWLSWTAQGFLPPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLE AADTVESLGNPGHEEFLPRGDRYDAIRACIGNTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTGREK GMVTVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAAEATLKINPQLKIDAHLNKVCPATESIYSDEFYTK QDIIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEIIVPQLTESYNSHRDPPEEEIPFCTLKSF PAAIEHTIQWARDKFESSFSHKPSLFNKFWQAYPSAEDVLQKIQNGQSLEGCFQVIKLLSRRPRIWSQCV ELARLKFEKYFNHKALQLLHCFPLETRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQSAAKLYATVYC IPFSEKDLSVNSLMDILSEVKIEEFKPSNKVVQTDETARKPDHVPVSSEDERNAVFQLEEALSSNKATKS DLQMTVLSFEKDDDRNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATSTAAVSGLVALEMI KVAGGYPFDAYKNCFLNLAIPIIVFTETSEVRKTEIRNGISFTIWDRWTVHGKEDFTLSDFINAVKENYG IEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPSTEKKYVDLTVSFAPDADGDEDLPGPPVRYYFSHD TNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	118 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_766300
Locus ID	231380
UniProt ID	Q8C7R4
Refseq Size	5101
Cytogenetics	5 E1
Refseq ORF	3162
Synonyms	4930542H01; 5730469D23Rik; AU021846; AW124799; E1-L2; Ube1l2
Summary	Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34 (By similarity). Essential for embryonic development. Required for UBD/FAT10 conjugation.[UniProtKB/Swiss-Prot Function]

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