2A mature peptide (NC_001489) Virus Tagged ORF Clone
CAT#: VC102185
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for 2A mature peptide [Hepatitis A virus], codon optimized for human cell expression, NP_740553
View other clones from "Virus" (10)
Need custom modification / cloning service?
Get a free quote
CNY 3,800.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC102185 represents NCBI reference of NP_740553 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCATATTGAGTGCCGGAAGCCCTACAAGGAACTTAGACTCGAGGTGGGGAAGCAGAGACTGAAAT ATGCCCAGGAGGAGCTTAGTAATGAGGTGCTCCCGCCTCCACGCAAGATGAAGGGACTGTTTTCCCAGGC CAAGATCTCCTTGTTTTATACGGAAGAACACGAAATAATGAAGTTCAGTTGGCGAGGGGTCACGGCAGAC ACAAGAGCCCTCAGACGCTTTGGCTTTAGCCTCGCTGCTGGCCGGAGCGTGTGGACATTGGAGATGGACG CCGGTGTCCTTACGGGCCGGCTGATCCGCCTGAACGACGAGAAGTGGACAGAGATGAAGGATGATAAGAT AGTAAGTCTGATTGAGAAGTTCACCTCCAACAAGTATTGGTCAAAAGTGAATTTTCCCCATGGCATGTTG GATCTGGAAGAAATCGCAGCTAATAGTAAAGATTTCCCTAACATGAGTGAAACCGACTTGTGTTTCCTGC TCCATTGGCTGAACCCAAAGAAGATAAACTTGGCGGATAGGATGCTGGGCCTGAGCGGTGTGCAGGAGAT CAAAGAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102185 representing NP_740553
Red=Cloning sites Green=Tags MSHIECRKPYKELRLEVGKQRLKYAQEELSNEVLPPPRKMKGLFSQAKISLFYTEEHEIMKFSWRGVTAD TRALRRFGFSLAAGRSVWTLEMDAGVLTGRLIRLNDEKWTEMKDDKIVSLIEKFTSNKYWSKVNFPHGML DLEEIAANSKDFPNMSETDLCFLLHWLNPKKINLADRMLGLSGVQEIKEQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001489 |
ORF Size | 570 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NC_001489.1, NP_740553 |
RefSeq ORF | 570 bp |
MW | 22.1 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
VC102180 | Myc-DDK-tagged ORF clone of viral ORF for 1A VP4a mature peptide (alt) [Hepatitis A virus], codon optimized for human cell expression, NP_740548 |
CNY 3,800.00 |
|
VC102181 | Myc-DDK-tagged ORF clone of viral ORF for 1A VP4b mature peptide (alt) [Hepatitis A virus], codon optimized for human cell expression, NP_740549 |
CNY 3,800.00 |
|
VC102182 | Myc-DDK-tagged ORF clone of viral ORF for 1B VP2 mature peptide [Hepatitis A virus], codon optimized for human cell expression, NP_740550 |
CNY 3,800.00 |
|
VC102183 | Myc-DDK-tagged ORF clone of viral ORF for 1C VP3 mature peptide [Hepatitis A virus], codon optimized for human cell expression, NP_740551 |
CNY 3,840.00 |
|
VC102184 | Myc-DDK-tagged ORF clone of viral ORF for 1D VP1 mature peptide [Hepatitis A virus], codon optimized for human cell expression, NP_740552 |
CNY 3,840.00 |
|
VC102186 | Myc-DDK-tagged ORF clone of viral ORF for 2B mature peptide [Hepatitis A virus], codon optimized for human cell expression, NP_740554 |
CNY 3,800.00 |
|
VC102187 | Myc-DDK-tagged ORF clone of viral ORF for 2C mature peptide [Hepatitis A virus], codon optimized for human cell expression, NP_740555 |
CNY 3,990.00 |
|
VC102188 | Myc-DDK-tagged ORF clone of viral ORF for 3A mature peptide [Hepatitis A virus], codon optimized for human cell expression, NP_740556 |
CNY 3,800.00 |
|
VC102189 | Myc-DDK-tagged ORF clone of viral ORF for 3B (VPg) mature peptide [Hepatitis A virus], codon optimized for human cell expression, NP_740557 |
CNY 3,800.00 |
|
VC102190 | Myc-DDK-tagged ORF clone of viral ORF for 3C mature peptide [Hepatitis A virus], codon optimized for human cell expression, NP_740558 |
CNY 3,800.00 |
其它2A mature peptide产品