CDC2L6 (CDK19) (NM_001300960) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA346C16.3; CDC2L6; CDK11; DEE87; EIEE87
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336240 representing NM_001300960. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATTATGATTTCAAGGCGAAGCTGGCGGCGGAGCGGGAGCGGGTGGAGGATTTGTTTGAGTACGAA GGGTGCAAAGTGGGACGCGGCACCTACGGTCACGTCTACAAGGCGAGGCGGAAAGATGGAAAAGATGAA AAGGAATATGCATTGAAGCAAATTGAAGGCACAGGAATATCCATGTCGGCTTGTAGAGAGATTGCACTT TTGCGAGAATTGAAGCACCCTAATGTGATTGCATTGCAGAAGGTGTTCCTTTCTCACAGTGACAGGAAG GTATGGCTGCTGTTTGATTATGCAGAGCATGACTTGTGGCATATTATTAAGTTTCACCGTGCATCAAAA GCAAATAAAAAGCCCATGCAGTTGCCAAGATCTATGGTTAAATCCTTACTTTACCAGATTCTTGATGGT ATCCATTACCTCCATGCAAATTGGGTGCTTCACAGAGACTTGAAACCAGCAAATATCCTAGTAATGGGA GAAGGTCCTGAGAGGGGGAGAGTCAAAATAGATATATGGGCAATAGGTTGTATATTTGCTGAATTGTTG ACTTCGGAACCTATTTTTCACTGTCGTCAGGAAGATATAAAAACAAGCAATCCCTTTCATCATGATCAA CTGGATCGGATATTTAGTGTCATGGGGTTTCCTGCAGATAAAGACTGGGAAGATATTAGAAAGATGCCA GAATATCCCACACTTCAAAAAGACTTTAGAAGAACAACGTATGCCAACAGTAGCCTCATAAAGTACATG GAGAAACACAAGGTCAAGCCTGACAGCAAAGTGTTCCTCTTGCTTCAGAAACTCCTGACCATGGATCCA ACCAAGAGAATTACCTCGGAGCAAGCTCTGCAGGATCCCTATTTTCAGGAGGACCCTTTGCCAACATTA GATGTATTTGCCGGCTGCCAGATTCCATACCCCAAACGAGAATTCCTTAATGAAGATGATCCTGAAGAA AAAGGTGACAAGAATCAGCAACAGCAGCAGAACCAGCATCAGCAGCCCACAGCCCCTCCACAGCAGGCA GCAGCCCCTCCACAGGCGCCCCCACCACAGCAGAACAGCACCCAGACCAACGGGACCGCAGGTGGGGCT GGGGCCGGGGTCGGGGGCACCGGAGCAGGGTTGCAGCACAGCCAGGACTCCAGCCTGAACCAGGTGCCT CCAAACAAGAAGCCACGGCTAGGGCCTTCAGGCGCAAACTCAGGTGGACCTGTGATGCCCTCGGATTAT CAGCACTCCAGTTCTCGCCTGAATTACCAAAGCAGCGTTCAGGGATCCTCTCAGTCCCAGAGCACACTT GGCTACTCTTCCTCGTCTCAGCAGAGCTCACAGTACCACCCATCTCACCAGGCCCACCGGTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300960
Insert Size	1377 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300960.1
RefSeq Size	6114 bp
RefSeq ORF	1377 bp
Locus ID	23097
UniProt ID	Q9BWU1
Protein Families	Druggable Genome, Protein Kinase
MW	51.8 kDa
Gene Summary	This gene encodes a protein that is one of the components of the Mediator co-activator complex. The Mediator complex is a multi-protein complex required for transcriptional activation by DNA binding transcription factors of genes transcribed by RNA polymerase II. The protein encoded by this gene is similar to cyclin-dependent kinase 8 which can also be a component of the Mediator complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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