C2orf65 (M1AP) (NM_001281295) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C2orf65; D6Mm5e; SPATA37; SPGF48
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335637 representing NM_001281295. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCATCCTGGGCGAACTACTGGTAAAGGGCCCTCTACTCACACTCAGATTGACCAGCAACCTCCACGG CTTCTCATTGTGCACATTGCTCTACCGTCCTGGGCTGACATCTGCACCAACCTCTGTGAGGCTCTGCAG AACTTCTTCTCTCTAGCCTGCAGCTTGATGGGCCCCAGCCGCATGTCCCTGTTCAGTTTATACATGGTA CAAGATCAGCATGAGTGCATCCTCCCTTTTGTGCAAGTGAAAGGGAACTTTGCTAGGTTGCAGACCTGC ATCTCAGAACTCCGCATGTTACAGAGAGAAGGGTGTTTCAGATCACAAGGTGCTTCTCTGCGGCTGGCA GTAGAGGATGGGCTCCAGCAATTCAAACAATACAGCAGACATGTGACCACAAGGGCAGCTCTGACCTAT ACCTCCCTGGAGATTACTATTCTGACTTCTCAGCCTGGAAAAGAGGTGGTCAAACAGTTGGAGGAAGGG TTGAAAGATACAGACCTAGCCAGAGTCAGGAGGTTTCAGGTCGTTGAGGTCACAAAGGGAATCCTAGAG CACGTGGACTCAGCGTCTCCTGTTGAGGATACCAGCAATGATGAGAGTTCTATTCTGGGAACTGACATT GACCTTCAGACTATAGACAATGATATCGTCAGCATGGAGATTTTCTTCAAAGCCTGGCTACATAACAGT GGAACAGACCAAGAACAAATCCATCTTCTTCTTTCTTCACAGTGTTTCAGCAACATTTCCAGACCCAGA GATAATCCAATGTGTCTGAAATGTGATCTCCAAGAGCGACTGCTCTGCCCATCCCTACTCGCTGGCACA GCTGACGGCTCCTTGAGAATGGATGACCCTAAAGGAGACTTCATCACACTCTACCAGATGGCTTCCCAG TCATCGGCCTCTCATTACAAGCTCCAAGTGATCAAGGCTTTAAAATCTAGCGGGCTCTGCGAGTCATTG ACATATGGACTCCCGTTCATCCTCAGACCTACAAGCTGTTGGCAGCTGGACTGGGATGAGCTGGAGACA AATCAGCAACATTTCCATGCTTTGTGTCACAGCCTGCTGGTGAGTACCCATGTCCCCAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281295
Insert Size	1098 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281295.1
RefSeq Size	1394 bp
RefSeq ORF	1098 bp
Locus ID	130951
UniProt ID	Q8TC57
MW	41 kDa
Gene Summary	This gene encodes a protein that is likely to function in progression of meiosis. A similar protein in mouse plays a role in gametogenesis in both sexes. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks several exons at its 3' end, and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus compared to isoform 1.

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