SEC14 like protein 2 (SEC14L2) (NM_001291932) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C22orf6; SPF; TAP; TAP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335523 representing NM_001291932. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTCCGGAAGCATGTGGAGTTCCGAAAGCAAAAGGACATTGACAACATCATTAGCTGGCAGCCTCCA GAGGTGATCCAACAGTATCTGTCAGGGGGTATGTGTGGCTATGACCTGGATGGCTGCCCAGTCTGGTAC GACATAATTGGACCTCTGGATGCCAAGGGTCTGCTGTTCTCAGCCTCCAAACAGGACCTGCTGAGGACC AAGATGCGGGAGTGTGAGCTGCTTCTGCAAGAGTGTGCCCACCAGACCACAAAGTTGGGGAGGAAGGTG GAGACCATCACCATAATTTATGACTGCGAGGGGCTTGGCCTCAAGCATCTCTGGAAGCCTGCTGTGGAG GCCTATGGAGAGTTTCTCTGCATGTTTGAGGAAAATTATCCCGAAACACTGAAGCGTCTTTTTGTTGTT AAAGCCCCCAAACTGTTTCCTGTGGCCTATAACCTCATCAAACCCTTCCTGAGTGAGGACACTCGTAAG AAGATCATGGTCCTGGGAGCAAATTGGAAGGAGGTTTTACTGAAACATATCAGCCCTGACCAGGTGCCT GTGGAGTATGGGGGCACCATGACTGACCCTGATGGAAACCCCAAGTGCAAATCCAAGATCAACTACGGG GGTGACATCCCCAGGAAGTATTATGTGCGAGACCAGGTGAAACAGCAGTATGAACACAGCGTGCAGATT TCCCGTGGCTCCTCCCACCAAGTGGAGTATGAGATCCTCTTCCCTGGCTGTGTCCTCAGGTGGCAGTTT ATGTCAGATGGAGCGGATGTTGGTTTTGGGATTTTCCTGAAGACCAAGATGGGAGAGAGGCAGCGGGCA GGGGAGATGACAGAGGTGCTGCCCAACCAGAGGTACAACTCCCACCTGGTCCCTGAAGATGGGACCCTC ACCTGCAGTGATCCTGGCATCTATGTCCTGCGGTTTGACAACACCTACAGCTTCATTCATGCCAAGAAG GTCAATTTCACTGTGGAGGTCCTGCTTCCAGACAAAGCCTCAGAAGAGAAGATGAAACAGCTGGGGGCA GGCACCCCGAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291932
Insert Size	1050 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291932.1
RefSeq Size	4228 bp
RefSeq ORF	1050 bp
Locus ID	23541
Protein Families	Transcription Factors
MW	39.9 kDa
Gene Summary	This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008] Transcript Variant: This variant (4) lacks an alternate exon and begins translation at a downstream start codon compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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