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PHF8 (NM_001184896) Human Untagged Clone
CAT＃: SC329271
PHF8 (untagged)-Human PHD finger protein 8 (PHF8) transcript variant 1
CNY 20,430.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | JHDM1F; KDM7B; MRXSSD; ZNF422 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC329271 representing NM_001184896.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACAGGAGCCGCGCTATCGTCCAGAGAGGACGCGTGCTGCCGCCTCCCGCCCCTCTTGACACGACG AACCTGGCCGGCCGCAGAACGCTCCAGGGCCGAGCGAAGATGGCCTCGGTGCCGGTGTATTGCCTCTGC CGGCTGCCTTACGATGTGACCCGCTTCATGATCGAGTGTGACATGTGCCAGGACTGGTTTCATGGCAGT TGTGTTGGTGTTGAAGAGGAGAAGGCTGCTGACATTGACCTCTACCACTGCCCCAACTGTGAAGTCTTG CATGGGCCCTCCATTATGAAAAAACGCCGTGGATCTTCAAAGGGGCATGATACACACAAGGGGAAACCA GTGAAGACCGGGAGCCCTACGTTCGTCAGAGAGCTCCGGAGTAGGACTTTTGACAGCTCAGATGAAGTG ATTCTGAAGCCCACTGGAAATCAACTGACCGTGGAATTCCTGGAAGAAAATAGCTTCAGTGTGCCCATC CTGGTCCTGAAGAAGGATGGGTTGGGCATGACGCTGCCCTCGCCATCATTCACTGTGAGGGATGTTGAA CACTATGTTGGTTCTGACAAAGAGATTGATGTGATTGATGTGACCCGCCAGGCTGACTGCAAGATGAAG CTTGGTGATTTTGTGAAATACTATTACAGCGGGAAGAGGGAGAAAGTCCTCAATGTCATTAGTTTGGAA TTCTCTGATACCAGACTTTCTAACCTTGTGGAGACACCGAAGATTGTTCGAAAGCTGTCATGGGTCGAA AACTTGTGGCCAGAGGAATGTGTCTTTGAGAGACCCAATGTACAGAAGTACTGCCTCATGAGTGTGCGA GATAGCTATACAGACTTTCACATTGACTTTGGTGGCACCTCTGTCTGGTACCATGTACTCAAGGGTGAA AAGATCTTCTACCTGATCCGCCCAACAAATGCCAATCTGACTCTCTTTGAGTGCTGGAGCAGTTCCTCT AATCAGAATGAGATGTTCTTTGGGGACCAGGTGGACAAGTGCTACAAGTGTTCCGTGAAGCAAGGACAG ACACTTTTCATTCCCACAGGGTGGATCCATGCTGTGCTGACGCCTGTGGACTGCCTTGCCTTTGGAGGG AACTTCTTACACAGCCTTAACATCGAGATGCAGCTCAAAGCCTATGAGATTGAGAAGCGGCTGAGCACA GCAGACCTCTTCAGATTCCCCAACTTTGAGACCATCTGTTGGTATGTGGGAAAGCACATCCTGGACATC TTTCGCGGTTTGCGAGAGAACAGGAGACACCCTGCCTCCTACCTGGTCCATGGTGGCAAAGCCTTGAAC TTGGCCTTTAGAGCCTGGACAAGGAAAGAAGCTCTGCCAGACCATGAGGATGAGATCCCGGAGACAGTG CGAACCGTACAGCTCATTAAAGATCTGGCCAGGGAGATCCGCCTGGTGGAAGACATCTTCCAACAGAAC GTTGGGAAGACGAGCAATATCTTTGGGCTGCAGAGGATCTTCCCAGCCGGCTCCATTCCCCTAACCAGG CCAGCCCATTCCACTTCAGTGTCCATGTCCAGGCTGTCACTGCCCTCCAAAAATGGTTCAAAGAAGAAA GGCCTGAAGCCCAAGGAACTCTTCAAGAAGGCAGAGCGAAAGGGCAAGGAGAGTTCAGCCTTGGGGCCT GCTGGCCAGTTGAGCTATAATCTCATGGACACATACAGTCATCAGGCACTGAAGACAGGCTCTTTCCAG AAAGCAAAGTTCAACATCACTGGTGCCTGCTTGAATGACTCAGATGACGACTCACCAGACTTGGACCTT GATGGAAATGAGAGCCCATTGGCCCTATTGATGTCTAACGGCAGTACGAAAAGGGTGAAGAGTTTATCC AAATCTCGGCGAACCAAGATAGCAAAGAAGGTAGACAAGGCTAGGCTGATGGCAGAACAGGTGATGGAA GACGAATTTGACTTGGATTCAGATGATGAGCTGCAGATTGACGAGAGATTGGGAAAGGAGAAGGCGACC CTGATAATAAGACCAAAATTTCCCCGGAAATTGCCCCGTGCGAAGCCTTGCTCTGACCCCAACCGAGTT CGTGAACCAGGAGAAGTTGAGTTTGACATTGAGGAGGACTATACAACAGATGAGGACATGGTGGAAGGG GTTGAAGGCAAGCTTGGGAATGGTAGTGGCGCTGGTGGCATTCTTGATCTGCTCAAGGCCAGCAGGCAG GTGGGGGGACCTGACTATGCTGCCCTCACCGAGGCCCCAGCTTCTCCCAGCACTCAGGAGGCCATCCAG GGCATGCTGTGCATGGCCAACCTGCAGTCCTCATCGTCCTCACCGGCTACCTCTAGCCTGCAGGCCTGG TGGACTGGGGGACAGGATCGAAGCAGTGGGAGCTCCAGCAGTGGGCTGGGCACAGTGTCTAACAGTCCT GCTTCCCAGCGCACCCCAGGGAAGCGGCCCATCAAGCGGCCAGCATACTGGAGAACCGAGAGCGAGGAG GAGGAGGAGAACGCCAGTCTGGATGAACAGGACAGCTTGGGAGCGTGCTTCAAGGATGCAGAGTATATC TATCCTTCACTGGAGTCTGATGATGATGACCCTGCTTTGAAATCTCGACCCAAGAAAAAGAAGAATTCA GATGATGCTCCATGGAGTCCTAAAGCCCGCGTGACCCCAACTCTGCCGAAGCAGGACCGTCCTGTGCGT GAGGGGACCCGGGTAGCCTCTATTGAGACAGGTTTGGCTGCAGCAGCTGCAAAGCTGGCCCAGCAGGAG CTACAGAAGGCCCAAAAGAAGAAATATATCAAGAAGAAGCCTTTGCTGAAGGAGGTAGAACAGCCTCGC CCTCAAGACTCCAATCTCAGTCTGACAGTACCAGCCCCCACTGTGGCTGCCACACCACAACTTGTCACC TCCTCCTCACCCCTGCCTCCTCCTGAGCCTAAACAAGAGGCCCTGTCAGGAAGTCTCGCTGACCATGAG TACACCGCTCGTCCCAATGCCTTTGGCATGGCCCAGGCAAACCGCAGCACCACACCTATGGCCCCCGGT GTCTTCTTGACCCAGCGGCGCCCTTCAGTTGGCTCCCAGAGCAATCAGGCAGGACAAGGAAAGCGTCCC AAAAAGGGCCTGGCCACAGCAAAGCAGAGACTCGGCCGTATCCTGAAAATCCACAGAAATGGCAAACTA CTTCTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001184896 |
| Insert Size | 3183 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001184896.1 |
| RefSeq Size | 5921 bp |
| RefSeq ORF | 3183 bp |
| Locus ID | 23133 |
| UniProt ID | Q9UPP1 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 117.9 kDa |
| Gene Summary | The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230633 | PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 11,072.00 |
|
| RC230633L1 | Lenti-ORF clone of PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 12,450.00 |
|
| RC230633L2 | Lenti-ORF clone of PHF8 (mGFP-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 13,472.00 |
|
| RC230633L3 | Lenti-ORF clone of PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 12,450.00 |
|
| RC230633L4 | Lenti-ORF clone of PHF8 (mGFP-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 12,450.00 |
|
| RG230633 | PHF8 (tGFP-tagged) - Human PHD finger protein 8 (PHF8), transcript variant 1 |
CNY 11,590.00 |
