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NEK11 (NM_024800) Human Untagged Clone
CAT＃: SC323687
NEK11 (untagged)-Kinase deficient mutant (K61M) of Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 1
CNY 4,808.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323687 sequence for NM_024800 edited (data generated by NextGen Sequencing)
ATGCTGAAATTCCAAGAGGCAGCTAAGTGTGTGAGTGGATCAACAGCCATTTCCACTTAT CCAAAGACCTTGATTGCAAGAAGATACGTGCTTCAACAAAAACTTGGCAGTGGAAGTTTT GGAACTGTCTATCTGGTTTCAGACAAGAAAGCCAAACGAGGAGAGGAATTAAAGGTACTT ATGGAAATATCTGTTGGAGAACTAAATCCAAATGAAACTGTACAGGCCAATTTGGAAGCC CAACTCCTCTCCAAGCTGGACCACCCAGCCATTGTCAAGTTCCATGCAAGTTTTGTGGAG CAAGATAATTTCTGCATTATCACGGAGTACTGTGAGGGCCGAGATCTGGACGATAAAATT CAGGAATATAAACAAGCTGGAAAAATCTTTCCAGAAAATCAAATAATAGAATGGTTTATC CAGCTGCTGCTGGGAGTTGACTACATGCATGAGAGGAGGATACTTCATCGAGACTTAAAG TCAAAGAATGTATTTCTGAAAAATAATCTCCTTAAAATTGGAGATTTTGGAGTTTCTCGA CTTCTAATGGGATCCTGTGACCTGGCCACAACTTTAACTGGAACTCCCCATTATATGAGT CCTGAGGCTCTGAAACACCAAGGCTATGACACAAAGTCGGACATCTGGTCACTGGCATGC ATTTTGTATGAGATGTGCTGCATGAATCATGCATTCGCTGGCTCCAATTTCTTATCCATT GTTTTAAAAATTGTTGAAGGTGACACACCTTCTCTCCCTGAGAGATATCCAAAAGAACTA AATGCCATCATGGAAAGCATGTTGAACAAGAATCCTTCATTAAGACCATCTGCTATCGAA ATTTTAAAAATCCCTTACCTTGATGAGCAGCTACAGAACCTAATGTGTAGATATTCAGAA ATGACTCTGGAAGACAAAAATTTGGATTGTCAGAAGGAGGCTGCTCATATAATTAATGCC ATGCAAAAAAGGATCCACCTGCAGACTCTGAGGGCACTGTCAGAAGTACAGAAAATGACG CCAAGAGAAAGGATGCGGCTGAGGAAGCTCCAGGCGGCTGATGAGAAAGCCAGGAAGCTG AAAAAGATTGTGGAAGAAAAATATGAAGAAAATAGCAAACGAATGCAAGAATTGAGATCT CGGAACTTTCAGCAGCTGAGTGTTGATGTACTCCATGAAAAAACACATTTAAAAGGAATG GAAGAAAAGGAGGAGCAACCTGAGGGAAGACTTTCTTGTTCACCCCAGGACGAGGATGAA GAGAGGTGGCAAGGCAGGGAAGAGGAATCTGATGAACCAACTTTAGAGAACCTGCCTGAG TCTCAGCCTATTCCTTCCATGGACCTCCACGAACTTGAATCAATTGTAGAGGATGCCACA TCTGACCTTGGATACCATGAGATCCCAGAAGACCCACTTGTGGCTGAAGAGTACTACGCT GATGCATTTGATTCCTATTGTGTAGAGAGTGATGAGGAGGAAGAAGAAATAGCGTTAGAA AGACCAGAGAAAGAAATCAGGAATGAGGGATCCCAGCCTGCTTACAGAACAAACCAACAG GACAGTGATATCGAAGCGTTGGCCAGGTGTTTGGAAAATGTCCTGGGTTGCACTTCTCTA GACACAAAGACCATCACCACCATGGCTGAAGACATGTCCCCAGGACCACCAATTTTCAAC AGTGTGATGGCCAGGACCAAGATGAAACGCATGAGGGAATCAGCCATGCAGAAGCTGGGG ACAGAAGTATTTGAAGAGGTCTATAATTACCTCAAGAGAGCAAGGCATCAGAATGCTAGC GAAGCAGAGATCCGCGAGTGTTTGGAAAAAGTGGTGCCTCAAGCCAGCGACTGTTTTGAA GTGGACCAGCTCCTGTACTTTGAAGAGCAGTTGCTGATCACGATGGGAAAAGAACCTACT CTCCAGAACCATCTCTAG Clone variation with respect to NM_024800.4 182 a=>t;1463 a=>t >OriGene 5' read for mutant NM_024800 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTTAATCTCATCTT TAAAATAAGGAGAATTACTGAGTGACCTGAAGGACCCTTTTCAGCTGGAAAGTCTGAACTGACCAACACT GGATGAATTTGACCATTTCTTAGGAGACTGGAATGTTAAGTTTCTATAAATGAATGAACCAGTTCTCTCT TGTTTGGAGCAATGCTGAAATTCCAAGAGGCAGCTAAGTGTGTGAGTGGATCAACAGCCATTTTCCACTT ATCCCAAAGACCTTGATTTGCAAGAAGATTACGTGCTTCACCAAAAAACTTGGCAGTTGGAAAGTTTTTG GACCTGGTCCTATCTGTTTTCAGAACAAGAAAAGCAAAACGGAGGAAGAGGATTTAAAGGTACTTTATGA AAAATATCTTGTGGAGGAACTAAAATCAAAATGAAAACTGGACCGGGCCATTTGGAAGCCCCACCTCTCT TTCCAACCGGGCACCCCCAGCCAATGGGCCAGGTTCCATTCCAGGTTTGTGGGGAACAAGATTATTTCGG CTATACCCGAAACCGGTAGGGGCCGAGTCTCGGGGAGATAAATTTCGGATTACAACTGGAAAACTTCCGA ATACAAATAGAAGGGTAACCCTCGCTGGATTGCACTCTCGAGAGAGAGACTCTCTCAGACTTAGTCCAGA GAGTTTTGAGATAATCCCTAATGGATTGAGTTTCGCATCTAGATCTGACTGCCACACTACTGATCCATAA GTCAGGCTGACAGTTACATGACTGACGAGCATGGGAGGTGCTGTATGCCTGTCAAACTTAACGTCAAGTG AGAAG >SC323687 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CAACTTGGCAGTGGAGTTTGGACTGTCTATCTGGTTTCAGACAAGAAAGCCAAACGAGGAGAGGAATTAA AGGTACTTATGGAAATATCTGTTGGAGAACTAAATCCAAATGAAACTGTACAGGCCAATTTGGAAGCCCA ACTCCTCTCCAAGCTGGACCACCCAGCCATTGTCAAGTTCCATGCAAGTTTTGTGGAGCAAGATAATTTC TGCATTATCACGGAGTACTGTGAGGGCCGAGATCTGGACGATAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_024800 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024800.2, NP_079076.2 |
| RefSeq Size | 2939 bp |
| RefSeq ORF | 1938 bp |
| Locus ID | 79858 |
| UniProt ID | Q8NG66 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009] Transcript Variant: This variant (1) encodes isoform (1), also known as NEK11L. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221953 | NEK11 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 1 |
CNY 4,560.00 |
|
| RC221953L1 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC221953L2 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC221953L3 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC221953L4 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG221953 | NEK11 (tGFP-tagged) - Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 1 |
CNY 5,040.00 |
|
| SC111981 | NEK11 (untagged)-Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 1 |
CNY 4,808.00 |
